University Copenhagen (Copenhagen, Denmark)

The research of the Anatomy and Stem Cell group at University Copenhagen is focused on embryology and iPSC generation. Several iPSC lines from AD and FTD patients have been generated at UCPH using an episomal based reprogramming methods. Subsequently these iPSC lines are differentiated into neural subtypes, which are predominately affected in AD and FTD. Disease phenotypes are studied in these neurons including for example mitochondria functional tests , assessment of autophagy activity, response to oxidative stress, abnormal cellular structures via TEM and protein aggregations.

Responsible Scientists:
Poul Hyttel, DVM, PhD, DVSc, Dhc. Professor, Stemmad project coordinator
Jørgen Erik Nielsen, MD, PhD. 
Kristine Freude, PhD. assistant professor. Stemmad scientific project manager
Troels Tolstrup Nielsen, PhD, Postdoctoral Research Scientist
Karina Garnier Christensen, PhD, Project Coordinator. Stemmad project manager
Karen Brackley, PhD, Project Coordinator. Stemmad project manager
Adele Marthaler, PhD, Experienced Researcher 4-10 recruitment.
Anna Poon, PhD, Experienced Researcher 4-10 recruitment.

Bioneer A/S (Hørsholm, Denmark)

Bioneer has participated in the generation of iPSC from AD, FTD and SCA2. The primary focus at Bioneer is the establishment of isogenic controls for the patient cell lines. The generation of isogenic controls is achieved via the CrisprCas9 technique in combination with ssODNs carrying the corrected sequences. The same technique is also used to generate homozygous mutations in order to enhance the cellular pathological phenotype.

Responsible Scientists:
Christian Clausen, PhD. Group Leader
Bjørn Holst, PhD. Principal Scientist

BioTalentum Ltd. (Gödöllő, Hungary)

Biotalentum’s task within the STEMMAD project:

-          Differentiation of iPSC into NPCs and neurons in 2D and 3 (contribution to WP2)
-          Providing complementary training
-          Contribution to dissemination activity

For the derivation of the neural cell models, the goal is to obtain several neuronal subtypes, representing the cell types primarily affected by the diseases, through in vitro differentiation of iPSCs from patients and healthy control individuals as well as genetically modified iPSC lines. Hence, the focus will be to obtain basal forebrain cholinergic neurons, and glutamatergic and gabaergic neurons of the forebrain, to analyze the cell type specific effects of the given disease.

Responsible Scientists:
Andras Dinnyes, VM, PhD, Professor and CEO,         
Sara Berzsenyi, PhD, Postdoctoral Resrach Scientist,
Csilla Nemes, PhD, Postdoctoral Resrach Scientist,
Juliana Kobolak, PhD, Deputy Director of Research,         

University of Szeged (Szeged, Hungary)

The main research profile of the Alzheimer’s Disease group at the Department of Medical Chemistry, University of Szeged is to study the molecular mechanisms underlying the neurodegenerative diseases associated with protein misfolding, and drug design for the therapy of the diseases. In the STEMMAD project our task is to examine the neuronal functions of the NPCs and neurons derived from iPSCs by using ex vivo hippocampal slice method for spike and fEPSP recordings. Putative drug molecules are also planned to be tested on the cell lines produced by the consortium members.

Responsible Scientists:
Livia Fülöp, PhD, Group Leader
Viktor Szegedi, PhD, Research Scientist
Gábor Juhász, PhD, Research Scientist
Edina Varga, PhD student

Mahidol University (Bangkok, Thailand)

Our current research is to apply stem cell technology to disease modeling and therapeutic applications, especially for neurodegenerative diseases. We are establishing patient-specific induced pluripotent stem (iPS) cells and reprogramming neural cells from other cell types of patients with neurological disorders. We aim to explore cellular and molecular mechanisms that underlie the neural degeneration and dissect the regulatory process of neural specification. Our long-term goal is to translate these models for drug discovery and to rebuild aging brain as for personalized medicine.

Responsible Scientists:
Narisorn Kitiyanant, DVM, PhD. Instructor
Yindee Kitiyanant, DVM, MSc. Professor
Objoon Trachoo, MD, PhD. Instructor
Pirut Tong-ngam, MSc. Technician
Phetcharat Phanthong. PhD student
Panetha Pansri. PhD student